Fundamentals and applications of single-molecule FRET in biophysical research
biomolecular structure and dynamics in vitro and in vivo[1]. I here introduce the principles of the technique with an overview of state-of-the-art applications[1,2], highlighting its role in assessing fundamental [...] different fluorescence imaging modalities including confocal-, video- and super-resolution microscopy[4]. [1] Lerner et al., Science 359 (2018) eaan1133 [2] Agam et al., Nature Methods 20 (2023) 523-535 [3] Han …
